Post-traumatic Stress Disorder,Social Support,and Quality of Life in Patients with Spinal Cord Injury / 中国医学科学院学报

Objective To explore the relationship among post-traumatic stress disorder(PTSD),social support and quality of life in patients with spinal cord injury(SCI)after a long recovery in China and investigate the factors influencing the quality of life. Methods In this cross-sectional study,206 SCI patients who were hospitalized in 9 hospitals were enrolled.Data collection was performed using general information,the Post-Traumatic Stress Disorder Checklist-Civilian version,the social support scales,and the World Health Organization Quality of Life-Abbreviated version.The data were statistically analyzed using

Association of FokI rs2228570 and TMPRSS6 rs855791 polymorphisms with cow's milk protein allergy in children / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To study the association of polymorphisms of FokI rs2228570 in the vitamin D receptor (VDR) gene and TMPRSS6 rs855791 with cow's milk protein allergy (CMPA) in children.</p><p><b>METHODS</b>Quantitative real-time PCR was used to analyze the single nucleotide polymorphisms of FokI rs2228570 in the VDR gene and TMPRSS6 rs855791 in 100 children with CMPA and 100 healthy children (control group). The multivariate logistic regression model was used to identify the risk factors for CMPA.</p><p><b>RESULTS</b>There were significant differences in the frequencies of CC, CT, and TT genotypes of TMPRSS6 rs855791 between the CMPA and control groups (P=0.008), and the CMPA group had a significantly higher frequency of TT genotype. The multivariate logistic regression analysis showed that the children with TT genotype of rs855791 had an increased risk of CMPA (OR=3.473, P=0.011). However, there was no significant difference in the genotype distribution of FokI rs2228570 in the VDR gene between the two groups (P=0.686).</p><p><b>CONCLUSIONS</b>TMPRSS6 rs855791 polymorphism is associated with CMPA in children, and TT genotype may be the susceptible genotype of CMPA. FokI rs2228570 polymorphism is not associated with CMPA.</p>

The clinical value of selection of the puncture path in performing CT-guided pericardial drainage / 介入放射学杂志

Objective To discuss the optimalselection of the puncture path in performing CT-guided pericardial drainage,and to evaluate its clinical feasibility and safety.Methods A total of 114 patients with pericardial effusion,who were admitted to authors' hospital during the period from May 2013 to March 2016,were enrolledin this study.The appropriate body position and suitable needle-puncturing route were selected,and CT-guided pericardial drainage with Seldinger'stechnique was performed.Results Successful puncturing and catheter drainage was obtained in all 114 patients,no any serious complication occurred.The time used for manipulation was 18-30 min.Conclusion The use of right puncture path is of great importance for the performance of CT-guided pericardial drainage for pericardial effusion,this technique is highly feasible and safe for relieving the clinical symptoms of pericardial tamponade.

CT-guided chemoablation in treating lumbar disc herniation :a randomized controlled study / 重庆医学

Objective To investigate the clinical effectiveness of chemoablation for the treatment of lumbar disc herniations (LDH) to provide reference for its clinical treatment .Methods A total of 191 cases of LDH were divided into the group A and B . All cases were performed the CT-guided chemoablation for treating LDH .The group A conducted the intra-disc injection treatment , while the group B conducted the intra-disc and extra-disc combined injection .The cases were followed up by the modes of outpatient department or telephone on postoperative 30 ,90 ,180 d .The follow up and statistical analysis were conducted by referring to the Macnab effect evaluation criteria .Results The two groups conducted the outpatient department follow up or telephone follow up on postoperative 30 ,90 ,180 d .All cases did not appear the complications such as nerve damage ,spinal cord damage and intervertebral disc infection .In the intra-group comparison ,the VAS score had statistical difference between before and after treatment ( P<0 .05) .In the inter-group comparison ,the score after treatment in the group B was lower than that in the group A (P<0 .05) .The good effect rate on postoperative 30 d in the group A was 81 .1% (77/95) ,which on postoperative 90 d was 83 .2% (79/95) and which on postoperative 180 d was 85 .3% (81/95) .The good effect rate in the group B was 85 .4% (82/96) on 30 d ,88 .5% (85/96) on 90 d and 91 .7% (88/96) on 180 d .Conclusion CT-guided lumbar intervertebral disc minimally invasive interventional chemoab-lation has good and rapid analgesic effect ,and the intra-disc and inter-disc combined treatment has better curative effect .

Evaluation of white matter myelination in preterm infants using DTI and MRI / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To investigate the features of white matter myelin development in preterm infants using magnetic resonance imaging (MRI) and diffusion tensor imaging (DTI).</p><p><b>METHODS</b>A total of 31 preterm infants with a gestational age of ≤32 weeks and a birth weight of <1 500 g were enrolled. According to head MRI findings, these infants were divided into preterm group with brain injury (12 infants) and preterm group without brain injury (19 infants). A total of 24 full-term infants were enrolled as control group. Head MRI and DTI were performed at a gestational age or corrected gestational age of 37-40 weeks. Fractional anisotropy (FA) and apparent diffusion coefficient (ADC) were measured for the same regions of interest in the three groups.</p><p><b>RESULTS</b>The preterm group with brain injury showed a significantly lower FA value of the posterior limb of the internal capsule than the preterm group without brain injury and full-term control group (P<0.05). The preterm groups with and without brain injury showed significantly lower FA values of frontal white matter and lenticular nucleus than the full-term control group (P<0.05). The FA value of occipital white matter showed no significant differences among the three groups (P>0.05). Compared with the full-term control group, the preterm groups with and without brain injury showed significantly higher ADC values of the posterior limb of the internal capsule, lenticular nucleus, occipital white matter, and frontal white matter (P<0.05).</p><p><b>CONCLUSIONS</b>After brain injury, preterm infants tend to develop disorder or delay of white matter myelination in the posterior limb of the internal capsule. At a corrected full-term gestational age, the preterm infants with and without brain injury have a lower grade of maturity in periventricular white matter and grey matter than full-term infants.</p>

Mechanical Strain Regulates Osteoblast Proliferation Through Ca-CaMK-CREB Signal Pathway / 中国医学科学杂志(英文版)

Objective To investigate the effects of mechanical strain on Ca-calmodulin dependent kinase (CaMK)-cAMP response element binding protein (CREB) signal pathway and proliferation of osteoblasts.Methods Using a four-point bending device, MC3T3-E1 cells were exposed to mechanical tensile strains of 2500 µs and 5000 µs at 0.5 Hz respectively. The intracellular free Ca([Ca]i) concentration and calmodulin activity were assayed by fluorospectrophotometry, CaMK II β, CREB, and phosphorylated (activated) CREB (p-CREB) were assessed by Western blot, and cells proliferation was assayed with MTT. Pretreatment with verapamil was carried out to block Cachannel, and inhibitor U73122 was used to inhibit phospholipase C (PLC).Results Mechanical strains of 2500 µs and 5000 µs for 1 to 10 minutes both increased [Ca]i level of the cells. The 2500 µs strain, a periodicity of 1 h/d for 3 days, activated calmodulin, elevated protein levels of CaMK II β and p-CREB, and promoted cells proliferation, which were attenuated by pretreatment of verapamil or U73122. The effects of 5000 µs strain on calmodulin, CaMK II β, p-CREB and proliferation were contrary to 2500 µs strain.Conclusion The mechanical strain regulates osteoblasts proliferation through Ca-CaMK-CREB signal pathway via Cachannel and PLC/IPtransduction cascades.

Clinical study of astragalus's preventing the recurrence of asthma in children / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the clinical efficacy of astragalus's preventing the recurrence and regulatory effects on Th1/Th2 cytokines in asthmatic children during the remission stage.</p><p><b>METHODS</b>Ninety asthmatic children during the remission stage were assigned to the astragalus treatment group (Group A), the hormone treatment group (Group B), and the combined group of astragalus and hormone treatment (Group C), 30 in each. Thirty healthy children were set up as the control group. The changes of peak expiratory flow rate (PEFR) before and after treatment and the recurrence times during the one-year follow-up were observed. Peripheral serum contents of immunoreactive fibronectin-gamma (IFN-gamma) and interleukin-4 (IL-4) were detected before and after treatment using ELISA.</p><p><b>RESULTS</b>The total effective rate was higher in Group B (73.3%) than in Group A (66.7%), but with no statistical difference between the two groups (P>0.05). It was highest in Group C (96.7%), showing significant difference from the other two groups (P<0.05). The levels of PEFR and IFN-gamma significantly increased and IL-4 obviously decreased in the three groups after treatment (P<0.05). No statistical difference of PEFR, IFN-gamma, or IL-4 existed in the three groups before treatment (P>0.05). Statistical difference of PEFR, IFN-gamma, or IL-4 existed between Group C and Group B after treatment (P<0.05).</p><p><b>CONCLUSIONS</b>Astragalus played a role in preventing the recurrence of asthma. The combination of astragalus and hormones showed better effects.</p>

Effect of vascular endothelial growth factor small interfering RNA (siRNA) on retinal microvascular endothelial cells under hypoxia condition in vitro / 中华儿科杂志

<p><b>OBJECTIVE</b>To explore VEGF siRNA's effect on the immature fetal retinal microvascular endothelial cells in vitro.</p><p><b>METHOD</b>The fresh retinal micrangium was primarily cultured to obtain microvascular endothelial cells. CoCl2 was used to simulate oxygen-deficient conditions. siRNA directed against human VEGF was designed and chemically synthesized. There were 3 groups in our experiment: VEGF siRNA group, hypoxia control group, and negative siRNA control group. The fetal retinal micrangium vascular endothelial cells were transfected by using liposome. The expression levels of VEGF mRNA and protein were evaluated by RT-PCR and Western blotting 24, 48, 72 h after transfection, cell proliferation was evaluated by MTT method.</p><p><b>RESULT</b>The expression levels of VEGF mRNA decreased by 21.05%, 79.67%, and 90.48% 24 h, 48 h, and 72 h after transfection as compared to those in hypoxia control group, the expression level of VEGF protein had decreased by 14.58%, 66.97%, and 81.61% as compared to those in hypoxia control group. The siRNA could decrease cell proliferation under hypoxia too, the multiplication rate after 12, 24, 48, and 72 h decreased by 15.0%, 42.9%, 78.3% and 65.9%.</p><p><b>CONCLUSION</b>VEGF siRNA could down-regulate the expression of VEGF in immature fetal retinal microvascular endothelial cells and suppressed cell proliferation. Application of siRNA to inhibit expression of VEGF may be a hopeful way to prevent and cure ROP.</p>

Estrogen receptor gene polymorphisms and HBV-induced liver cirrhosis / 中南大学学报(医学版)

OBJECTIVE@#To investigate the relationship between estrogen receptor (ER) gene polymorphisms and HBV-induced cirrhosis.@*METHODS@#Xba I and Pvu II polymorphisms of ER gene were analyzed in 98 patients with HBV-induced cirrhosis, 72 patients with chronic hepatitis B, and 84 healthy controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.@*RESULTS@#The frequencies of Pp genotype and P allele of ER gene in patients with HBV-induced cirrhosis were higher than those in patients with chronic hepatitis B and controls, while the frequencies of pp genotype and p allele of ER gene in patients with HBV-induced cirrhosis were lower than those in patients with chronic hepatitis B and controls (P 0.05).@*CONCLUSION@#Pp genotype and P allele might be the susceptibility gene for HBV-induced cirrhosis while pp genotype and p allele might be the protective gene.

Expression and action of vascular endothelial growth factor and its receptor in a rat model of retinopathy of prematurity / 中华儿科杂志

<p><b>OBJECTIVE</b>To explore the expression of vascular endothelial growth factor (VEGF) and its receptors (flt-1 and flk-1) in the retina of retinopathy of prematurity (ROP), and its relation to the alteration of retinal blood vessels.</p><p><b>METHODS</b>Eighty-six newborn Sprague-Dawley rats were randomly divided into hyperoxia and air groups, then each group was further divided into 1, 3, 7 and 14 days subgroups. The rats in hyperoxia group inhaled 75% oxygen and ROP model was thus set up. These animals were sacrificed respectively after 1, 3, 7 and 14 days, then the retinal endothelial cells were marked by CD34 to observe the change of retinal blood vessels. The expression of VEGF, flt-1 and flk-1 in the retina was measured by immunohistochemistry.</p><p><b>RESULTS</b>The retinal capillary density index (RCDI) in control group increased as days went on (F = 21.589, P < 0.01, but it was the least on the 7th day in hyperoxia group, after the rats had been returned to air for 7 days, RCDI increased significantly (F = 67.885, P < 0.01); In the control group, the expression of VEGF and flk-1 was the strongest in the retina on the 7th day, the result had significant difference as compared with the 1st and 14th day (P < 0.05). The expression of VEGF and flk-1 on the 7th day in hyperoxia group was weaker than that of control group (P < 0.05). But on the 14th day in hyperoxia group, they were stronger than that of control (P < 0.05). The localization of the expression of flt-1 was changed when blood vessels altered, but there was no significant difference in expression intensity as a whole (P > 0.05).</p><p><b>CONCLUSION</b>When the premature retina was exposed to hyperoxia, the expression of VEGF and flk-1 was reduced, and retinal blood vessels were also decreased; but the expression of VEGF and flk-1 was stronger in retina when premature rats were exposed to relative hypoxia, and the retinal blood vessels also increased significantly. It is concluded that VEGF and flk-1 may play important roles in the development of retinal blood vessels and its change in ROP. However, flt-1 has less effect compared with flk-1.</p>